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Edta Trypsin Proteomics Grade Tpck Trypsin Recombinant Trypsin

Categories Recombinant Trypsin
Brand Name: YaxinBio
Model Number: RPT0201
Certification: ISO9001
Place of Origin: China
MOQ: 10mg
Price: 65$/10mg
Payment Terms: T/T
Packaging Details: ice packaging
Supply Ability: 1000g per week
Delivery Time: 8 days
Manufacturer: YaxinBio
Packaging: 10mg,100mg,1g or bulk
Source: E. Coli
Physical form: White lyophilized powder
Related Product: Recombinant Human Trypsin
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    Edta Trypsin Proteomics Grade Tpck Trypsin Recombinant Trypsin

    Edta Trypsin Proteomics Grade Tpck Trypsin Recombinant Trypsin


    Description


    Enzyme Commission (EC) Number: 3.4.21.4
    Molecular Weight: 23.3 kDa (bovine & porcine)
    Extinction Coefficient: E1% = 12.9 - 15.4 (280 nm)
    pI: 10.1 - 10.5(bovine)

    Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end

    of lysine and arginine amino acid residues. The pH optimum of trypsin is pH 7.0 - 9.0. The enzyme

    is inhibited by serine protease inhibitors, e.g. PMSF, aprotinin, soybean trypsin inhibitor and by

    metal chelating agents, e.g., EDTA.

    Recombinant Porcine Trypsin is a genetically engineered protein expressed in E.coli and purified

    by high pressure liquid chromatography. There are no any other contaminating enzyme activities.

    No protease inhibitors such as PMSF are contained in the preparation.


    Advantages


    Animal origin free:The use of recombinant porcine trypsin eliminates the risk of virus presence, and other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Porcine Trypsin belongs to the AOF level 3.

    Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.

    High purity:

    1) Recombinant porcine trypsin provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes.

    2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.


    Main Features


    Purified byHPLC
    Specific activity≥3800 USP units/mg pro
    Purity(RP-HPLC)NLT 70% β-trysin, NMT 20% α-trypsin
    Contaminant activityNo chymotrypsin, carboxypeptidase A, and other protease contaminant.

    Unit Definition:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute

    in a reaction volume of 3.0ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).


    Recommend Usage


    To prepare 1-10mg/ml recombinant trypsin with 1mM HCl. The ratio to aimed protein is 1:50 to

    1:1000 (w/w), the optimum pH is pH7-10.


    Storage And Stability


    Recommend recombinant trypsin lyophilized powder stored under 2-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.

    Product Tags:

    porcine trypsin

      

    cell culture trypsin

      
    Quality Edta Trypsin Proteomics Grade  Tpck Trypsin  Recombinant Trypsin for sale
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